Detection of Atmospheric Peracids Using HPLC Chemiluminescent Method


Heather Hart
Argonne National Laboratory
Mentor: Jeff Gaffney

Peracids may play important roles as oxidants in air pollution. They are highly reactive and therefore difficult to study. Traditional methods of measuring peracids often take too long and lack accuracy. We are developing the first HPLC (high-pressure liquid chromatography) method using chemiluminescent detection to separate and measure the different peracids found in the atmosphere. This method will allow instantaneous measurements of the peracid group. The Peracid Chemiluminescent Detection (PCD) instrument is currently under construction. We plan to take the sample directly from the air by collecting the peracids in a liquid trap, pumping the sample through a column, and each different peracid then reacts with luminol in a reaction cell upon elution, and the signal will be then recorded. In order to calibrate and make sure the HPLC works, we synthesized peroxyacetic acid, peroxypropionic acid, peroxybutyric acid, peroxymethacrylic acid, and peroxybenzoic acid. FTIR spectra will be taken of each of the synthesized peracids before they are run through the LC. The LC will be able to detect amounts of peracids at the ppb level, allowing us a better understanding of the peracid components in the atmosphere.

Presentation: Detection of Atmospheric Peracids Using HPLC Chemiluminescent Method


The Global Change Education Program is funded by the
U.S. Department of Energy

Office of Biological and Environmental Research